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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-59116 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Nicalin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicalin Antibody [NBP2-59116] - Detection of human and mouse NCLN by western blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody NBP2-59116 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicalin Antibody [NBP2-59116] - Detection of mouse NCLN by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody NBP2-59116. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nicalin Antibody [NBP2-59116] - Detection of human NCLN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCLN antibody NBP2-59116 (lot NBP2-59116-2) used for IP at 20 ul/mg lysate. NCLN was also immunoprecipitated by a previous lot of this antibody (lot NBP2-59116-1) and another rabbit anti-NCLN antibody. For blotting immunoprecipitated NCLN, NBP2-59116 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
