Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP2-61436 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal POU3F2/OCT7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human, mouse, rat POU3F2/OCT7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cerebral cortex tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in mouse cerebral cortex tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cell line U-251 MG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows absence of positivity in layer 6 neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows moderate nuclear positivity in neurons in layers 2-4.