Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-87396 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87396, RRID:AB_11025926
- Product name
- Rabbit Polyclonal SUN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SUN1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability.
The synaptonemal complex has liquid crystalline properties and spatially regulates meiotic recombination factors.
Muscular dystrophy-associated SUN1 and SUN2 variants disrupt nuclear-cytoskeletal connections and myonuclear organization.
A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton.
Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Samp1 is functionally associated with the LINC complex and A-type lamina networks.
Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7.
Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission.
Takaki T, Montagner M, Serres MP, Le Berre M, Russell M, Collinson L, Szuhai K, Howell M, Boulton SJ, Sahai E, Petronczki M
Nature communications 2017 Jul 24;8:16013
Nature communications 2017 Jul 24;8:16013
The synaptonemal complex has liquid crystalline properties and spatially regulates meiotic recombination factors.
Rog O, Köhler S, Dernburg AF
eLife 2017 Jan 3;6
eLife 2017 Jan 3;6
Muscular dystrophy-associated SUN1 and SUN2 variants disrupt nuclear-cytoskeletal connections and myonuclear organization.
Meinke P, Mattioli E, Haque F, Antoku S, Columbaro M, Straatman KR, Worman HJ, Gundersen GG, Lattanzi G, Wehnert M, Shackleton S
PLoS genetics 2014 Sep;10(9):e1004605
PLoS genetics 2014 Sep;10(9):e1004605
A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton.
Horn HF, Kim DI, Wright GD, Wong ES, Stewart CL, Burke B, Roux KJ
The Journal of cell biology 2013 Sep 30;202(7):1023-39
The Journal of cell biology 2013 Sep 30;202(7):1023-39
Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly.
Crabbe L, Cesare AJ, Kasuboski JM, Fitzpatrick JA, Karlseder J
Cell reports 2012 Dec 27;2(6):1521-9
Cell reports 2012 Dec 27;2(6):1521-9
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
Samp1 is functionally associated with the LINC complex and A-type lamina networks.
Gudise S, Figueroa RA, Lindberg R, Larsson V, Hallberg E
Journal of cell science 2011 Jun 15;124(Pt 12):2077-85
Journal of cell science 2011 Jun 15;124(Pt 12):2077-85
Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7.
Zwerger M, Kolb T, Richter K, Karakesisoglou I, Herrmann H
Molecular biology of the cell 2010 Jan 15;21(2):354-68
Molecular biology of the cell 2010 Jan 15;21(2):354-68
Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission.
Ramachandran R, Pucadyil TJ, Liu YW, Acharya S, Leonard M, Lukiyanchuk V, Schmid SL
Molecular biology of the cell 2009 Nov;20(22):4630-9
Molecular biology of the cell 2009 Nov;20(22):4630-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SUN1 Antibody [NBP1-87396] - Simple Western lane view shows a specific band for SUN1 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SUN1 Antibody [NBP1-87396] - Electropherogram image(s) of corresponding Simple Western lane view. SUN1 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87396 (A) shows similar protein distribution across tissues to independent antibody NBP1-87395 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.