TA504197

antibody from OriGene

Targeting: KCNJ3 GIRK1, KGA, Kir3.1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504197

Provider

OriGene

Proper citation

OriGene Cat#TA504197, RRID:AB_11126786

Product name

KCNJ3 (GIRK1 ) mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)

Antibody type

Monoclonal

Description

KCNJ3 (GIRK1 ) mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)

Host

Mouse

Conjugate

Unconjugated

Epitope

KCNJ3

Isotype

IgG

Antibody clone number

OTI1G3

Vial size

100 µl

Concentration

0.51 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCNJ3 (RC205322, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCNJ3.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-KCNJ3 mouse monoclonal antibody (TA504197) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCNJ3(RC205322).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504197)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504197)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504197)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504197)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504197)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205322 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KCNJ3 antibody(TA504197), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-KCNJ3 antibody(TA504197),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC