V3967

antibody from NSJ Bioreagents

Targeting: FAF1 CGI-03, hFAF1, HFAF1s, UBXD12, UBXN3A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Antibody Data

Product number

V3967

Provider

NSJ Bioreagents

Product name

FAF1 Antibody / FAS-associated factor 1

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

CPTC-FAF1-2

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)

Storage

Store the FAF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HEK293, HeLa and MCF-7 cell lysate with FAF1 antibody. Expected molecular weight ~74 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma with FAF1 antibody. HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer with FAF1 antibody. HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to staining.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FAF1 antibody (clone CPTC-FAF1-2). These results demonstrate the foremost specificity of the CPTC-FAF1-2 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.