AF2624

antibody from Novus Biologicals

Targeting: KLK7 PRSS6, SCCE

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2624

Provider

Novus Biologicals

Product name

Goat Polyclonal Kallikrein 7 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human and mouse Kallikrein 7 in Western blots. Detects human Kallikrein 7 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 4, rhKallikrein 5, rhKallikrein 8, rhKallikrein 9, rhKallikrein 11, rhKallikrein 13 and rhKallikrein 14 is observed.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

PRSS3/Mesotrypsin and kallikrein-related peptidase 5 are associated with poor prognosis and contribute to tumor cell invasion and growth in lung adenocarcinoma.

Ma H, Hockla A, Mehner C, Coban M, Papo N, Radisky DC, Radisky ES
Scientific reports 2019 Feb 12;9(1):1844

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Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice.

Kidana K, Tatebe T, Ito K, Hara N, Kakita A, Saito T, Takatori S, Ouchi Y, Ikeuchi T, Makino M, Saido TC, Akishita M, Iwatsubo T, Hori Y, Tomita T
EMBO molecular medicine 2018 Mar;10(3)

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Clinical value of protein expression of kallikrein-related peptidase 7 (KLK7) in ovarian cancer.

Dorn J, Gkazepis A, Kotzsch M, Kremer M, Propping C, Mayer K, Mengele K, Diamandis EP, Kiechle M, Magdolen V, Schmitt M
Biological chemistry 2014 Jan;395(1):95-107

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Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.

Muñoz-Repeto I, García MJ, Kamieniak M, Ramón Y Cajal T, Domingo S, Cazorla A, García Donas J, Hernando Polo S, García Sagredo JM, Hernández E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benítez J
Histology and histopathology 2013 Jan;28(1):133-44

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The extracellular matrix protein fibronectin is a substrate for kallikrein 7.

Ramani VC, Haun RS
Biochemical and biophysical research communications 2008 May 16;369(4):1169-73

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Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.

Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
Biological chemistry 2007 Nov;388(11):1235-42

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Kallikrein 7 by Western Blot. Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 7 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Kallikrein 7 in Human Skin. Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.