Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PAB4415 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4415, RRID:AB_1576706
- Product name
- KLK7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of KLK7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references LEKTI is localized in lamellar granules, separated from KLK5 and KLK7, and is secreted in the extracellular spaces of the superficial stratum granulosum.
KLK5 and KLK7, two members of the human tissue kallikrein family, are differentially expressed in lung cancer.
Genetic association between an AACC insertion in the 3'UTR of the stratum corneum chymotryptic enzyme gene and atopic dermatitis.
Ishida-Yamamoto A, Deraison C, Bonnart C, Bitoun E, Robinson R, O'Brien TJ, Wakamatsu K, Ohtsubo S, Takahashi H, Hashimoto Y, Dopping-Hepenstal PJ, McGrath JA, Iizuka H, Richard G, Hovnanian A
The Journal of investigative dermatology 2005 Feb;124(2):360-6
The Journal of investigative dermatology 2005 Feb;124(2):360-6
KLK5 and KLK7, two members of the human tissue kallikrein family, are differentially expressed in lung cancer.
Planque C, de Monte M, Guyetant S, Rollin J, Desmazes C, Panel V, LemariƩ E, Courty Y
Biochemical and biophysical research communications 2005 Apr 22;329(4):1260-6
Biochemical and biophysical research communications 2005 Apr 22;329(4):1260-6
Genetic association between an AACC insertion in the 3'UTR of the stratum corneum chymotryptic enzyme gene and atopic dermatitis.
Vasilopoulos Y, Cork MJ, Murphy R, Williams HC, Robinson DA, Duff GW, Ward SJ, Tazi-Ahnini R
The Journal of investigative dermatology 2004 Jul;123(1):62-6
The Journal of investigative dermatology 2004 Jul;123(1):62-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of KLK7 (arrow) using KLK7 polyclonal antibody (Cat # PAB4415).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KLK7 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed and paraffin embedded human skin was performed with KLK7 polyclonal antibody (Cat # PAB4415) at 1:10-1:50 dilution followed by indirect peroxidase conjugation with secondary antibody and DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells (right histogram), compared to a negative control cell (left histogram), was performed with KLK7 polyclonal antibody (Cat # PAB4415) and FITC-conjugated goat-anti-rabbit secondary antibody.
- Validation comment
- Flow Cytometry