HPA012072

antibody from Atlas Antibodies

Targeting: LASP1 Lasp-1, MLN50

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA012072

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA012072, RRID:AB_1852714

Product name

Anti-LASP1

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSV
VADTPELQRIKKTQDQISNIKYHEEFEKSRMGPSG
GEGMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPV
YQQPQQQPVAQSYGGYKE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

A complex containing LPP and α-actinin mediates TGFβ-induced migration and invasion of ErbB2-expressing breast cancer cells.

Ngan E, Northey JJ, Brown CM, Ursini-Siegel J, Siegel PM
Journal of cell science 2013 May 1;126(Pt 9):1981-91

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using Anti-LASP1 antibody. Corresponding LASP1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in cells in reaction center.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN