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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00004440-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004440-M04, RRID:AB_733220
- Product name
- MSI1 monoclonal antibody (M04), clone 3F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MSI1.
- Antigen sequence
METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TT
QEGLREYF GQFGE VKECL VMRDPLTKRS RGF
GFVTFMD QAGVDKVLAQ SRHELDS- Isotype
- IgG
- Antibody clone number
- 3F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells.
Lee HC, Ling QD, Yu WC, Hung CM, Kao TC, Huang YW, Higuchi A
Drug design, development and therapy 2013;7:491-502
Drug design, development and therapy 2013;7:491-502
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MSI1 monoclonal antibody (M04), clone 3F2 Western Blot analysis of MSI1 expression in IMR-32 ( Cat # L008V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MSI1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MSI1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to MSI1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
