14-5759-82
antibody from Invitrogen Antibodies
Targeting: SIGLEC7
CD328, p75/AIRM1, QA79, SIGLEC-7, SIGLEC19P, SIGLECP2
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 14-5759-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD328 (Siglec7) Monoclonal Antibody (eBioQA79 (QA79)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioQA79 monoclonal antibody reacts with adhesion inhibitory receptor molecule 1 (AIRM1), also known as sialic acid binding immunoglobulin-like lectin 7 (Siglec-7), p75 and CDw328. AIRM1 is a cell surface receptor that belongs to the sialoadhesin family and displays homology with the myeloid cell antigen CD33. p75/AIRM1 expression is confined to NK cells in lymphoid cells. eBioQA79 crosslinking inhibits NK function. p75/AIRM1 is expressed at various stages of myeloid differentiation. Presence of this protein is a useful way to monitor cellular proliferation and determine proliferative states of cells, especially in diseases such as chronic myeloid leukemia. Applications Reported: This eBioQA79 (QA79) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Applications Tested: This eBioQA79 (QA79) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioQA79 (QA79)
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation.
On-chip synthesis and screening of a sialoside library yields a high affinity ligand for Siglec-7.
Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells.
Kawasaki N, Rillahan CD, Cheng TY, Van Rhijn I, Macauley MS, Moody DB, Paulson JC
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1560-6
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1560-6
On-chip synthesis and screening of a sialoside library yields a high affinity ligand for Siglec-7.
Rillahan CD, Schwartz E, Rademacher C, McBride R, Rangarajan J, Fokin VV, Paulson JC
ACS chemical biology 2013 Jul 19;8(7):1417-22
ACS chemical biology 2013 Jul 19;8(7):1417-22
Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells.
Falco M, Biassoni R, Bottino C, Vitale M, Sivori S, Augugliaro R, Moretta L, Moretta A
The Journal of experimental medicine 1999 Sep 20;190(6):793-802
The Journal of experimental medicine 1999 Sep 20;190(6):793-802
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD328 (Siglec7) Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL