Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 11-1023-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-14-3-3 Antibody
- Antibody type
- Polyclonal
- Description
- YWHAQ, also known as 14-3-3 tau (14-3-3tau) is a a phosphoserine-binding protein and a member of the 14-3-3 family of proteins. The 14-3-3 family members are dimeric phosphoserine/ phosphothreonine-binding proteins, which are involved in a very diverse spectrum of signaling pathways. YWHAQ plays a critical role in the regulation of E2F1 stability. It binds to Ser31-phosphorylated E2F1 and inhibits the ubiquitination of E2F1 during DNA damage. The binding of YWHAQ to E2F1 interferes with the function of an E31 ligase and therefore inhibits the ubiquitination and degradation of E2F1. This interaction is required for the expression and induction of several E2F1 apoptotic target genes as well as apoptosis during DNA damage.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
A partial length recombinant 14-3-3
protein (amino acids 20-225) was u
sed as the immunogen for this antib
ody.- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freezing and thawing
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Supportive validation
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- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3. Anti-14-3-3 antibody (11-1023) was used at 4 µg/ml on (1) MOLT-4 and (2) MCF-7 lysates.