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LearnH00005473-M01
antibody from Novus Biologicals
Targeting: PPBP
b-TG1, Beta-TG, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, NAP-2-L1, PBP, SCYB7, TGB, TGB1, THBGB1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00005473-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005473-M01, RRID:AB_2166349
- Product name
- Mouse Monoclonal CXCL7/NAP-2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PPBP - pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Expression of chemokines CXCL4 and CXCL7 by synovial macrophages defines an early stage of rheumatoid arthritis.
Yeo L, Adlard N, Biehl M, Juarez M, Smallie T, Snow M, Buckley CD, Raza K, Filer A, Scheel-Toellner D
Annals of the rheumatic diseases 2016 Apr;75(4):763-71
Annals of the rheumatic diseases 2016 Apr;75(4):763-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi ( Cat # H00005473-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 (Cat # H00005473-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Detection limit for recombinant GST tagged PPBP is approximately 0.03ng/ml as a capture antibody.
