Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2130 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Siglec-10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) Siglec-5 is observed and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-3, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q96LC7- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells.
Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells.
Xie J, Christiaens I, Yang B, Trus I, Devriendt B, Cui T, Wei R, Nauwynck HJ
Veterinary research 2018 Jul 18;49(1):67
Veterinary research 2018 Jul 18;49(1):67
Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells.
Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS
Allergy 2006 Jun;61(6):769-76
Allergy 2006 Jun;61(6):769-76
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Siglec-10 in Human B Cells by Flow Cytometry. Human whole blood CD19+ B cells were stained with Goat Anti-Human Siglec-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2130, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phy-coerythrin-conjugated Anti-Goat IgG Secondary Anti-body (Catalog # F0107).
