IC36081G

antibody from R&D Systems

Targeting: PRDM1 BLIMP1, PRDI-BF1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC36081G

Provider

R&D Systems

Product name

Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human BLIMP1/PRDM1 in Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antigen sequence

O75626


Isotype

IgG

Antibody clone number

646702

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.

Bankó Z, Pozsgay J, Szili D, Tóth M, Gáti T, Nagy G, Rojkovich B, Sármay G
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 15;198(4):1512-1520

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Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis.

Rao DA, Gurish MF, Marshall JL, Slowikowski K, Fonseka CY, Liu Y, Donlin LT, Henderson LA, Wei K, Mizoguchi F, Teslovich NC, Weinblatt ME, Massarotti EM, Coblyn JS, Helfgott SM, Lee YC, Todd DJ, Bykerk VP, Goodman SM, Pernis AB, Ivashkiv LB, Karlson EW, Nigrovic PA, Filer A, Buckley CD, Lederer JA, Raychaudhuri S, Brenner MB
Nature 2017 Feb 1;542(7639):110-114

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MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets.

Kurioka A, Ussher JE, Cosgrove C, Clough C, Fergusson JR, Smith K, Kang YH, Walker LJ, Hansen TH, Willberg CB, Klenerman P
Mucosal immunology 2015 Mar;8(2):429-40

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC36081G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of BLIMP1/PRDM1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19⁺ CD3^- cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC36081G) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.