RQ6763

antibody from NSJ Bioreagents

Targeting: CDT1 DUP, RIS2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6763

Provider

NSJ Bioreagents

Product name

CDT1 Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the CDT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Jurkat, 2) human HepG2, 3) human Raji, 4) human K562, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with CDT1 antibody. Predicted molecular weight ~60 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HepG2, 2) human HeLa, 3) human 293T, 4) rat liver, 5) rat brain, 6) mouse liver and 7) mouse brain tissue lysate with CDT1 antibody. Predicted molecular weight ~60 kDa, commonly observed at 60-70 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with CDT1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with CDT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDT1 antibody.