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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX115461 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115461, RRID:AB_10731616
- Product name
- CaMKK beta antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Submitted references PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27(Kip1) axis.
Methyl cinnamate inhibits adipocyte differentiation via activation of the CaMKK2-AMPK pathway in 3T3-L1 preadipocytes.
Tuo L, Xiang J, Pan X, Hu J, Tang H, Liang L, Xia J, Hu Y, Zhang W, Huang A, Wang K, Tang N
Journal of experimental & clinical cancer research : CR 2019 Feb 4;38(1):50
Journal of experimental & clinical cancer research : CR 2019 Feb 4;38(1):50
Methyl cinnamate inhibits adipocyte differentiation via activation of the CaMKK2-AMPK pathway in 3T3-L1 preadipocytes.
Chen YY, Lee MH, Hsu CC, Wei CL, Tsai YC
Journal of agricultural and food chemistry 2012 Feb 1;60(4):955-63
Journal of agricultural and food chemistry 2012 Feb 1;60(4):955-63
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: MCF-7 7.5% SDS PAGE GTX115461 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX115461 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CaMKK beta antibody [N2C1], Internal (GTX115461) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed MCF-7, using CaMKK beta(GTX115461) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CaMKK beta(GTX115461) antibody at 1:500 dilution.
