bs-0237R

antibody from Bioss

Targeting: YWHAB YWHAA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-0237R

Provider

Bioss

Proper citation

Bioss Cat#bs-0237R, RRID:AB_10859172

Product name

14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic peptide derived from human 14-3-3

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha/Beta/Gamma/Delta/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 31kD. Observed band size: 31kD.

Sample type

Mouse

Other comments

Brain

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37°C

Sample type

Human

Other comments

Colon