Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-67833 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal 14-3-3 beta/alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded human breast tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded mouse brain tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of Hela cells with 14-3-3 alpha+beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.