AF4724

antibody from R&D Systems

Targeting: YWHAB YWHAA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4724

Provider

R&D Systems

Product name

Human/Mouse/Rat 14-3-3 beta Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat 14-3-3 beta in Western blots. Does not cross-react with other recombinant human 14-3-3 isoforms.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P31946


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/ Rat 14-3-3 beta by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line cells, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat 14-3-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recom-binant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (20 ng/lane) were included. A specific band for 14-3-3 beta was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.