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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [8]
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- Product number
- LS-C313483 - Provider product page
- Provider
- LSBio
- Product name
- SFN / Stratifin / 14-3-3 Sigma Antibody (aa140-156) LS-C313483
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Horse, Porcine, Rabbit, Sheep, Simian
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of SFN / Stratifin / 14-3-3 Sigma antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: A549 Cell Lysate. Lane 4: COLO320 Cell Lysate. Lane 5: SE620 Cell Lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-14-3-3 sigma antibody, Western blotting All lanes: Anti 14-3-3 sigma at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ug Lane 2: A549 Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: COLO320 Whole Cell Lysate at 40ug Lane 5: SE620 Whole Cell Lysate at 40ug Predicted bind size: 28KD Observed bind size: 28KD
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Esophagus Squama Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Esophagus Squama Cancer Tissue.
