RQ5791

antibody from NSJ Bioreagents

Targeting: ACADSB ACAD7, SBCAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5791

Provider

NSJ Bioreagents

Product name

ACADSB Antibody / SBCAD

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the ACADSB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human A431, 2) human T-47 D, 3) human A549, 4) monkey COS-7, 5) rat liver and 6) mouse liver lysate with ACADSB antibody. Expected molecular weight: ~47 kDa (unprocessed), ~43 kDa (processed).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with ACADSB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human ThP-1 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse RAW264.7 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat RH-35 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.