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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA500733 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500733, RRID:AB_11140529
- Product name
- Anti-DPP4 (CD26) mouse monoclonal antibody, clone OTI11D7 (formerly 11D7)
- Antibody type
- Monoclonal
- Description
- Anti-DPP4 (CD26) mouse monoclonal antibody, clone OTI11D7 (formerly 11D7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DPP4
- Isotype
- IgG
- Antibody clone number
- OTI11D7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Angiotensin II Stimulation of DPP4 Activity Regulates Megalin in the Proximal Tubules.
Dipeptidyl Peptidase 4 Distribution in the Human Respiratory Tract: Implications for the Middle East Respiratory Syndrome.
Establishment of a dipeptidyl peptidases (DPP) 8/9 expressing cell model for evaluating the selectivity of DPP4 inhibitors.
Rapid generation of a mouse model for Middle East respiratory syndrome.
Aroor A, Zuberek M, Duta C, Meuth A, Sowers JR, Whaley-Connell A, Nistala R
International journal of molecular sciences 2016 May 20;17(5)
International journal of molecular sciences 2016 May 20;17(5)
Dipeptidyl Peptidase 4 Distribution in the Human Respiratory Tract: Implications for the Middle East Respiratory Syndrome.
Meyerholz DK, Lambertz AM, McCray PB Jr
The American journal of pathology 2016 Jan;186(1):78-86
The American journal of pathology 2016 Jan;186(1):78-86
Establishment of a dipeptidyl peptidases (DPP) 8/9 expressing cell model for evaluating the selectivity of DPP4 inhibitors.
Huan Y, Jiang Q, Liu JL, Shen ZF
Journal of pharmacological and toxicological methods 2015 Jan-Feb;71:8-12
Journal of pharmacological and toxicological methods 2015 Jan-Feb;71:8-12
Rapid generation of a mouse model for Middle East respiratory syndrome.
Zhao J, Li K, Wohlford-Lenane C, Agnihothram SS, Fett C, Zhao J, Gale MJ Jr, Baric RS, Enjuanes L, Gallagher T, McCray PB Jr, Perlman S
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 1;111(13):4970-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 1;111(13):4970-5
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP4 (RC209466, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of protein levels of DPP4/8/9 in 293E cells transfected with pcDNA3.1 (Mock), pcDNA3.1-hDPP8 (DPP8) and pcDNA3.1-hDPP9 (DPP9), the purified recombinant human DPP4 protein (rhDPP4) was used as a positive control for the DPP4 antibody, antibodies targeting human DPP4/8/9 (TA500733/TA504366/TA504040) and ?-actin (TA310155) were purchased from OriGene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-DPP4 mouse monoclonal antibody (TA500733) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4(RC209466).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-DPP4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500733, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry of DPP4 protein level by using anti-DPP4 antibody in lungs harvested from BALB/c mice at day 3. Dilution: 1:800
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-DPP4 antibody(TA500733),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
