PAB20211

antibody from Abnova Corporation

Targeting: IFT43 C14orf179, FLJ32173, MGC16028

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20211

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20211, RRID:AB_10966948

Product name

IFT43 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant IFT43.

Antigen sequence

EEVQEEDFVLQVAAPPSIQIKRVMTYRDLDNDLMK
YSAIQTLDGEIDLKLLTKVLAPEHEVREDDVGWDW
DHLFTEVSSEVLTEWDPLQTEKEDPA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with IFT43 polyclonal antibody (Cat # PAB20211) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with IFT43 polyclonal antibody (Cat # PAB20211) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)