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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA5-18118 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-II Spectrin Monoclonal Antibody (3D7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody contains enough material to conduct 10 mini-Western blots.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D7
- Vial size
- 100 µL
- Concentration
- ~1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Alpha II Spectrin in rat hippocampal lysate using a Alpha II Spectrin monoclonal antibody (Product # MA5-18118). Results show a band at ~240kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of SPTAN1 in rat hippocampal lysate showing specific immunolabeling of a ~240 kDa band corresponding to alpha-II Spectrin monoclonal antibody (Product # MA5-18118).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Alpha II Spectrin in cultured rat neurons and glia using a Alpha II Spectrin monoclonal antibody (Product # MA5-18118) showing axonal and dendritic staining of Alpha II Spectrin (green) revealing the submembraneous cytoskeleton and DNA (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SPTAN1 in neurons and glia cells showing specific axonal and dendritic labeling with alpha-II Spectrin monoclonal antibody (Product # MA5-18118) using a dilution of 1:500 (green), nuclear staining with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SPTAN1 in rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons, cell bodies, and dendrites of Purkinje cells with alpha-II Spectrin monoclonal antibody (Product # MA5-18118) using a dilution of 1:2,000 (green). Labeling the processes of Bergmann glia and astrocytes with anti-GFAP using a dilution of 1:5,000 (red).
