BM604S

antibody from Acris Antibodies GmbH

Targeting: B2M

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

BM604S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#BM604S, RRID:AB_973246

Product name

anti Beta-2-microglobulin

Antibody type

Monoclonal

Antigen

Purified Human beta-2-Microglobulin

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

B2M-01

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line. Lane 2: EL4 mouse lymphoblastic lymphoma cell line. Lane 3: U937 human histiocytic lymphoma cell line.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Confocal microscopy of Hman Beta-2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor® 647 conjugate and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2 µg/ml.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

1. Murine 4T1 cells were mixed with human DU-145 cells and stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Red stained cells mark the Beta-2-microglobulin positive human DU-145 cells.2. Murine 4T1 cells were stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Murine 4T1 cells are negative for Beta-2-microglobulin staining.Pictures by courtesy of Fraunhofer ITEM, Regensburg, Germany.