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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG10206 - Provider product page
- Provider
- Arigo
- Product name
- anti-beta 2 Microglobulin antibody [D2E9]
- Antibody type
- Monoclonal
- Antigen
- Highly purified beta 2 microglobulin from human urine
- Description
- Protein G affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- D2E9
- Vial size
- 100 µg
- Storage
- Store at -20°C. Storage in frost free freezers is not recommended. After Reconstitution, Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 1. B2MG antigen (100 ng) 2. Jurkat cell lysate stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) at 1µg/mL The molecular weight of B2MG is about 13-14KD
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Methanol-fixed HeLa cells stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) (green)
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) followed by incubation with fluorescence labelled secondary antibody.
