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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- BM604S - Provider product page
- Provider
- OriGene
- Product name
- beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B2M-01, Purified
- Antibody type
- Monoclonal
- Description
- beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B2M-01, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- B2M
- Isotype
- IgG
- Antibody clone number
- B2M-01
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human ?2-microglobulin . Lane 1: RAJI human Burkitt lymphoma cell line. Lane 2: EL4 mouse lymphoblastic lymphoma cell line. Lane 3: U937 human histiocytic lymphoma cell line.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal microscopy of Hman Beta-2-microglobulin-transfected canine cells labeled A) with antibody Alexa Fluor? 647 conjugate and B) with Alexa Fluor? 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2 ug/ml.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- 1. Murine 4T1 cells were mixed with human DU-145 cells and stained with 1 ug Beta-2-microglobulin antibody . Anti-mouse Cy3 was used as a secondary antibody. Red stained cells mark the Beta-2-microglobulin positive human DU-145 cells. 2. Murine 4T1 cells were stained with 1 ug Beta-2-microglobulin antibody . Anti-mouse Cy3 was used as a secondary antibody. Murine 4T1 cells are negative for Beta-2-microglobulin staining. Pictures by courtesy of Fraunhofer ITEM, Regensburg, Germany.
- Validation comment
- IHC
