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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB5428 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5428, RRID:AB_10546991
- Product name
- B2M monoclonal antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against B2M.
- Isotype
- IgG
- Storage
- Store at -20°C .After reconstitution with sterile PBS, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references The small subunit of HL-A antigens is beta 2-microglobulin.
Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids.
Grey HM, Kubo RT, Colon SM, Poulik MD, Cresswell P, Springer T, Turner M, Strominger JL
The Journal of experimental medicine 1973 Dec 1;138(6):1608-12
The Journal of experimental medicine 1973 Dec 1;138(6):1608-12
Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids.
Berggård I, Bearn AG
The Journal of biological chemistry 1968 Aug 10;243(15):4095-103
The Journal of biological chemistry 1968 Aug 10;243(15):4095-103
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- The membrane blot was probed with B2M monoclonal antibody (Cat # MAB5428) (1 ug/mL).Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.Arrows indicate cellular recombinant B2M (12 KDa).Lane 1. Marker.Lane 2. HeLa cell lysate (induced).Lane 3. MD231.Lane 4. U251.Lane 5. Purified B2M protein.
