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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-488-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Thrombopoietin/Tpo Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat Thrombopoietin/Tpo in direct ELISAs. Neutralizes the biological activity of recombinant mouse Thrombopoietin/Tpo. It will also neutralize the activity of recombinant human (rh) Thrombopoietin/Tpo, although 25 times the amount of Ig is required. In direct ELISAs less than 15% cross-reactivity with rhTpo is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Multicolor quantitative confocal imaging cytometry.
Thrombopoietin contributes to the formation and the maintenance of hematopoietic progenitor-containing cell clusters in the aorta-gonad-mesonephros region.
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Thrombopoietin contributes to the formation and the maintenance of hematopoietic progenitor-containing cell clusters in the aorta-gonad-mesonephros region.
Harada K, Nobuhisa I, Anani M, Saito K, Taga T
Cytokine 2017 Jul;95:35-42
Cytokine 2017 Jul;95:35-42
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Shih CC, Hu MC, Hu J, Weng Y, Yazaki PJ, Medeiros J, Forman SJ
Blood 2000 Mar 15;95(6):1957-66
Blood 2000 Mar 15;95(6):1957-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Mouse Thrombopoietin/Tpo Antibody. Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND50 is typically 0.1-0.3 µg/mL.
