500-P49BT-25UG

antibody from Invitrogen Antibodies

Targeting: THPO MGDF, MPLLG, TPO

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P49BT-25UG

Provider

Invitrogen Antibodies

Product name

Thrombopoietin Polyclonal Antibody, Biotin, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL.

Conjugate

Biotin

Concentration

0.1-1.0 mg/mL

References

Submitted references

Analysis of Jak2 signaling reveals resistance of mouse embryonic hematopoietic stem cells to myeloproliferative disease mutation.

Mascarenhas MI, Bacon WA, Kapeni C, Fitch SR, Kimber G, Cheng SW, Li J, Green AR, Ottersbach K
Blood 2016 May 12;127(19):2298-309

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hTPO by Western Blot analysis Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hTPO by Western Blot analysis Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Thrombopoietin Polyclonal Antibody (Product # 500-P49-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTPO.

Conjugate

Biotin