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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-288-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human Thrombopoietin/Tpo Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Tpo in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Tpo and recombinant rat Tpo is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P40225- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts.
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Scaffidi AK, Mutsaers SE, Moodley YP, McAnulty RJ, Laurent GJ, Thompson PJ, Knight DA
British journal of pharmacology 2002 Jul;136(5):793-801
British journal of pharmacology 2002 Jul;136(5):793-801
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64
Blood 2000 Jun 15;95(12):3758-64
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody. Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA). The ND50 is typically 0.05-0.3 µg/mL.
