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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- AF8480 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Eps15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Eps15 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P42566- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Eps15 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Eps15 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Eps15 in HeLa Human Cell Line. Eps15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to granules in the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Eps15 in NIH-3T3 Mouse Cell Line. Eps15 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
