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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-15581 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP3CB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references AKT inhibition-mediated dephosphorylation of TFE3 promotes overactive autophagy independent of MTORC1 in cadmium-exposed bone mesenchymal stem cells.
Pin1 mediates Aβ(42)-induced dendritic spine loss.
Pi H, Li M, Zou L, Yang M, Deng P, Fan T, Liu M, Tian L, Tu M, Xie J, Chen M, Li H, Xi Y, Zhang L, He M, Lu Y, Chen C, Zhang T, Wang Z, Yu Z, Gao F, Zhou Z
Autophagy 2019 Apr;15(4):565-582
Autophagy 2019 Apr;15(4):565-582
Pin1 mediates Aβ(42)-induced dendritic spine loss.
Stallings NR, O'Neal MA, Hu J, Kavalali ET, Bezprozvanny I, Malter JS
Science signaling 2018 Mar 20;11(522)
Science signaling 2018 Mar 20;11(522)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HT-29 (Lane 2), U-87 MG (Lane 3), SK-N-AS (Lane 4), IMR-32 (Lane 5), Jurkat (Lane 6), MOLT-4 (Lane 7), tissue extracts of Mouse Brain (Lane 8) and Rat Brain (Lane 9). The blot was probed with Anti-PPP3CB Polyclonal Antibody (Product # PA5-15581, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 59 kDa band corresponding to PPP3CB was observed across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse kidney tissue lysate using a PPP3CB polyclonal antibody (Product # PA5-15581).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PPP3CB polyclonal antibody (Product # PA5-15581) in Jurkat cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PPP3CB was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PPP3CB Polyclonal Antibody (Product # PA5-15581) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a PPP3CB polyclonal antibody (Product # PA5-15581), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
