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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX100986 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100986, RRID:AB_10616555
- Product name
- Amphiregulin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer.
Khelwatty S, Essapen S, Bagwan I, Green M, Seddon A, Modjtahedi H
Oncotarget 2017 Jan 31;8(5):7666-7677
Oncotarget 2017 Jan 31;8(5):7666-7677
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE GTX100986 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IMR32 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody (GTX100986) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody (GTX100986) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody (GTX100986) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Amphiregulin antibody detects Amphiregulin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Amphiregulin antibody (GTX100986) diluted at 1:750.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Amphiregulin antibody detects Amphiregulin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Amphiregulin antibody (GTX100986) diluted at 1:500.
