Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF989 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Amphiregulin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Amphiregulin in ELISAs and Western Blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P31955
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Deoxycholic acid activates epidermal growth factor receptor and promotes intestinal carcinogenesis by ADAM17-dependent ligand release.
A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation.
Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia.
Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway.
ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
Pubertal exposure to high fat diet causes mouse strain-dependent alterations in mammary gland development and estrogen responsiveness.
The Arf-inducing transcription factor Dmp1 encodes a transcriptional activator of amphiregulin, thrombospondin-1, JunB and Egr1.
Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice.
Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
Dong W, Liu L, Dou Y, Xu M, Liu T, Wang S, Zhang Y, Deng B, Wang B, Cao H
Journal of cellular and molecular medicine 2018 Sep;22(9):4263-4273
Journal of cellular and molecular medicine 2018 Sep;22(9):4263-4273
A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation.
Fujiu K, Shibata M, Nakayama Y, Ogata F, Matsumoto S, Noshita K, Iwami S, Nakae S, Komuro I, Nagai R, Manabe I
Nature medicine 2017 May;23(5):611-622
Nature medicine 2017 May;23(5):611-622
Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia.
Jiang WJ, Song HX, Li SY, Guo B, Wu JF, Li GP, Guo DD, Shi L, Bi HS, Jonas JB
EBioMedicine 2017 Mar;17:134-144
EBioMedicine 2017 Mar;17:134-144
Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway.
Taverna S, Pucci M, Giallombardo M, Di Bella MA, Santarpia M, Reclusa P, Gil-Bazo I, Rolfo C, Alessandro R
Scientific reports 2017 Jun 9;7(1):3170
Scientific reports 2017 Jun 9;7(1):3170
ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
Yang Y, Ahn YH, Chen Y, Tan X, Guo L, Gibbons DL, Ungewiss C, Peng DH, Liu X, Lin SH, Thilaganathan N, Wistuba II, Rodriguez-Canales J, McLendon G, Creighton CJ, Kurie JM
The Journal of clinical investigation 2014 Jun;124(6):2696-708
The Journal of clinical investigation 2014 Jun;124(6):2696-708
Pubertal exposure to high fat diet causes mouse strain-dependent alterations in mammary gland development and estrogen responsiveness.
Olson LK, Tan Y, Zhao Y, Aupperlee MD, Haslam SZ
International journal of obesity (2005) 2010 Sep;34(9):1415-26
International journal of obesity (2005) 2010 Sep;34(9):1415-26
The Arf-inducing transcription factor Dmp1 encodes a transcriptional activator of amphiregulin, thrombospondin-1, JunB and Egr1.
Mallakin A, Sugiyama T, Kai F, Taneja P, Kendig RD, Frazier DP, Maglic D, Matise LA, Willingham MC, Inoue K
International journal of cancer 2010 Mar 15;126(6):1403-16
International journal of cancer 2010 Mar 15;126(6):1403-16
Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice.
Murthy A, Defamie V, Smookler DS, Di Grappa MA, Horiuchi K, Federici M, Sibilia M, Blobel CP, Khokha R
The Journal of clinical investigation 2010 Aug;120(8):2731-44
The Journal of clinical investigation 2010 Aug;120(8):2731-44
Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
Monslow J, Sato N, Mack JA, Maytin EV
The Journal of investigative dermatology 2009 Aug;129(8):2046-58
The Journal of investigative dermatology 2009 Aug;129(8):2046-58
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human and Mouse Amphiregulin by Western Blot. Western blot shows 25 ng of Recombinant Mouse Amphiregulin (Catalog # 989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Amphiregulin in Mouse Skin. Amphiregulin was detected in immersion fixed frozen sections of mouse skin using Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Amphiregulin and Neutralization by Mouse Amphiregulin Antibody. Recombinant Mouse Amphiregulin (Catalog # 989-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND50 is typically 0.3-1.5 µg/mL.