Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12-5370-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Amphiregulin Monoclonal Antibody (AREG559), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AREG858 monoclonal antibody reacts with human Amphiregulin (AR). AR is a member of the Epidermal Growth Factor (EGF) family and is expressed as a transmembrane precursor protein and released by proteolytic cleavage. Soluble AR is a ligand for the EGF receptor (EGFR) and the interaction promotes growth of normal epithelial cells, fibroblasts and keratinocytes. In the context of the immune system AR functions as an effector molecule for tissue repair and homeostasis, mediates resistance and tolerance to infection, and suppresses inflammation. Under different disease and activating conditions AR is expressed in T cells, group 2 innate lymphoid cells, basophils, mast cells, eosinophils, and neutrophils. Applications Reported: This AREG559 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AREG559 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- AREG559
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood lymphocytes were stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) (purple histogram) overnight or only with Protein Transport Inhibitor Cocktail (Product # 00-4980) (orange histogram). Cells were fixed and stained intracellularly with Anti-Human CD4 PerCP-Cyanine5.5 (Product # 45-0049-42) and Anti-Human Amphiregulin PE using the Intracellular Fixation and Permeabilization Buffers set (Product # 88-8824). Viable, CD4+ T cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866), were used for analysis.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood lymphocytes were stimulated with Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975) overnight. Cells were fixed and stained intracellularly with Anti-Human CD3 PerCP-Cyanine5.5 (Product # 45-0036-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-82) (left) or Anti-Human Amphiregulin PE (Product # 12-5370) (right) using the Intracellular Fixation and Permeabilization Buffers set (Product # 88-8824). Viable cells the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866), were used for analysis.
- Conjugate
- Yellow dye