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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ7312 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AREG Antibody / Amphiregulin
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the AREG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A549 and 2) MCF7 cell lysate with AREG antibody. Molecular weight: 28 kDa (non-glycosylated), ~50 kDa (glycosylated pro form), ~43 kDa (predominant glycosylated soluble form) as well as other, smaller, soluble and membrane bound forms.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Caco-2 cells with AREG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AREG antibody.
