Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF262 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Amphiregulin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Amphiregulin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Amphiregulin, rmEpiregulin, recombinant human (rh) HB-EGF, and rhTGF-alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15514- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Deoxycholic acid activates epidermal growth factor receptor and promotes intestinal carcinogenesis by ADAM17-dependent ligand release.
Yes-associated protein promotes the abnormal proliferation of psoriatic keratinocytes via an amphiregulin dependent pathway.
Combinations of Bevacizumab and Erlotinib Show Activity in Colorectal Cancer Independent of RAS Status.
An integrative analysis of the tumorigenic role of TAZ in human non-small cell lung cancer.
Aberrantly activated AREG-EGFR signaling is required for the growth and survival of CRTC1-MAML2 fusion-positive mucoepidermoid carcinoma cells.
Human antigen R-mediated mRNA stabilization is required for ultraviolet B-induced autoinduction of amphiregulin in keratinocytes.
Expression of amphiregulin and EGFRvIII affect outcome of patients with squamous cell carcinoma of the head and neck receiving cetuximab-docetaxel treatment.
Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma.
Plasma-membrane-anchored growth factor pro-amphiregulin binds A-type lamin and regulates global transcription.
Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells.
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
Dong W, Liu L, Dou Y, Xu M, Liu T, Wang S, Zhang Y, Deng B, Wang B, Cao H
Journal of cellular and molecular medicine 2018 Sep;22(9):4263-4273
Journal of cellular and molecular medicine 2018 Sep;22(9):4263-4273
Yes-associated protein promotes the abnormal proliferation of psoriatic keratinocytes via an amphiregulin dependent pathway.
Jia J, Li C, Yang J, Wang X, Li R, Luo S, Li Z, Liu J, Liu Z, Zheng Y
Scientific reports 2018 Oct 15;8(1):14513
Scientific reports 2018 Oct 15;8(1):14513
Combinations of Bevacizumab and Erlotinib Show Activity in Colorectal Cancer Independent of RAS Status.
Mésange P, Bouygues A, Ferrand N, Sabbah M, Escargueil AE, Savina A, Chibaudel B, Tournigand C, André T, de Gramont A, Larsen AK
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Jun 1;24(11):2548-2558
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Jun 1;24(11):2548-2558
An integrative analysis of the tumorigenic role of TAZ in human non-small cell lung cancer.
Noguchi S, Saito A, Horie M, Mikami Y, Suzuki HI, Morishita Y, Ohshima M, Abiko Y, Mattsson JS, König H, Lohr M, Edlund K, Botling J, Micke P, Nagase T
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Sep 1;20(17):4660-72
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Sep 1;20(17):4660-72
Aberrantly activated AREG-EGFR signaling is required for the growth and survival of CRTC1-MAML2 fusion-positive mucoepidermoid carcinoma cells.
Chen Z, Chen J, Gu Y, Hu C, Li JL, Lin S, Shen H, Cao C, Gao R, Li J, Ha PK, Kaye FJ, Griffin JD, Wu L
Oncogene 2014 Jul 17;33(29):3869-77
Oncogene 2014 Jul 17;33(29):3869-77
Human antigen R-mediated mRNA stabilization is required for ultraviolet B-induced autoinduction of amphiregulin in keratinocytes.
Nakayama H, Fukuda S, Matsushita N, Nishida-Fukuda H, Inoue H, Shirakata Y, Hashimoto K, Higashiyama S
The Journal of biological chemistry 2013 Apr 12;288(15):10338-48
The Journal of biological chemistry 2013 Apr 12;288(15):10338-48
Expression of amphiregulin and EGFRvIII affect outcome of patients with squamous cell carcinoma of the head and neck receiving cetuximab-docetaxel treatment.
Tinhofer I, Klinghammer K, Weichert W, Knödler M, Stenzinger A, Gauler T, Budach V, Keilholz U
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Aug 1;17(15):5197-204
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Aug 1;17(15):5197-204
Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma.
Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 1;15(21):6529-40
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 1;15(21):6529-40
Plasma-membrane-anchored growth factor pro-amphiregulin binds A-type lamin and regulates global transcription.
Isokane M, Hieda M, Hirakawa S, Shudou M, Nakashiro K, Hashimoto K, Hamakawa H, Higashiyama S
Journal of cell science 2008 Nov 1;121(Pt 21):3608-18
Journal of cell science 2008 Nov 1;121(Pt 21):3608-18
Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells.
Eckstein N, Servan K, Girard L, Cai D, von Jonquieres G, Jaehde U, Kassack MU, Gazdar AF, Minna JD, Royer HD
The Journal of biological chemistry 2008 Jan 11;283(2):739-50
The Journal of biological chemistry 2008 Jan 11;283(2):739-50
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
American journal of respiratory cell and molecular biology 2007 Nov;37(5):578-88
American journal of respiratory cell and molecular biology 2007 Nov;37(5):578-88
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
Biology of reproduction 2007 Jul;77(1):53-60
Biology of reproduction 2007 Jul;77(1):53-60
Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A
The European respiratory journal 2007 Dec;30(6):1064-73
The European respiratory journal 2007 Dec;30(6):1064-73
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
Development (Cambridge, England) 2006 Feb;133(4):751-9
Development (Cambridge, England) 2006 Feb;133(4):751-9
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
Kansra S, Stoll SW, Johnson JL, Elder JT
Molecular biology of the cell 2004 Sep;15(9):4299-309
Molecular biology of the cell 2004 Sep;15(9):4299-309
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Amphiregulin by Western Blot. Western blot shows conditioned media from T47D human breast cancer cell line untreated (-) or treated (+) with PMA and PHA for 3 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Amphiregulin in MCF-7 Human Cell Line. Amphiregulin was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human Amphiregulin Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF262) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Amphiregulin in Human Breast. Amphiregulin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Amphiregulin and Neutralization by Human Amphiregulin Antibody. Recombinant Human Amphiregulin (Catalog # 262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262). The ND50 is typically 0.3-1 µg/mL.
