Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-24494 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Myeloid-Associated Differentiation Marker Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A quantitative proteomic approach for identification of potential biomarkers in hepatocellular carcinoma.
Chaerkady R, Harsha HC, Nalli A, Gucek M, Vivekanandan P, Akhtar J, Cole RN, Simmers J, Schulick RD, Singh S, Torbenson M, Pandey A, Thuluvath PJ
Journal of proteome research 2008 Oct;7(10):4289-98
Journal of proteome research 2008 Oct;7(10):4289-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Human spleen lysate in the 1) absence and 2) presence of immunizing peptide and 3) rat spleen lysate probed with MYADM antibody at 0.25 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Total protein from mouse spleen, rat spleen and human stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Myeloid Associated Protein in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Immunohistochemical labeling was performed on formalin-fixed, paraffin embedded liver tissue microarrays. Data show 6 different sections (3 each from non-cancerous and cancer tissues). The MYADM antibody was used at 1:400 dilution. (Data courtesy of Dr. Akhilesh Pandey's laboratory at the Johns Hopkins University).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Analysis of human tonsil using MYADM antibody at 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Flow analysis of AML cell line using Myeloid-Associated Differentiation Marker Antibody. Image from verified customer review.