A300-973A

antibody from Invitrogen Antibodies

Targeting: BPTF FAC1, FALZ, NURF301

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-973A

Provider

Invitrogen Antibodies

Product name

FALZ/BPTF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody (Product # A300-973A; lot A300-973A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody A300-973A (lot A300-973A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated FALZ/BPTF, A300-973A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody A300-973A (lot A300-973A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-FALZ/BPTF (Product # A300-973A; lot 3) used at a dilution of 1:1,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FALZ/BPTF Cat. No. A300-973A Lot1 used at a dilution of 1:250 (0.8µg/ml) (left) and Cat. No. A300-973A Lot2 used at a dilution of 1:1,000 (0.2µg/ml) (right). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody (Product # A300-973A; lot A300-973A-3) used for IP at 6 µg per reaction. FALZ/BPTF was also immunoprecipitated by a previous lot of this antibody (lot A300-973A-2). For blotting immunoprecipitated FALZ/BPTF (Product # A300-973A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.