A300-973A

antibody from Invitrogen Antibodies

Targeting: BPTF FAC1, FALZ, NURF301

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-973A

Provider

Invitrogen Antibodies

Product name

FALZ/BPTF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody (Product # A300-973A; lot A300-973A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody A300-973A (lot A300-973A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated FALZ/BPTF, A300-973A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody A300-973A (lot A300-973A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-FALZ/BPTF (Product # A300-973A; lot 3) used at a dilution of 1:1,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FALZ/BPTF Cat. No. A300-973A Lot1 used at a dilution of 1:250 (0.8µg/ml) (left) and Cat. No. A300-973A Lot2 used at a dilution of 1:1,000 (0.2µg/ml) (right). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody (Product # A300-973A; lot A300-973A-3) used for IP at 6 µg per reaction. FALZ/BPTF was also immunoprecipitated by a previous lot of this antibody (lot A300-973A-2). For blotting immunoprecipitated FALZ/BPTF (Product # A300-973A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.