Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NB100-41418 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-41418, RRID:AB_2067212
- Product name
- Rabbit Polyclonal BPTF/FALZ Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes.
Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z.
Xu W, Long L, Zhao Y, Stevens L, Felipe I, Munoz J, Ellis RE, McGrath PT
eLife 2019 Sep 9;8
eLife 2019 Sep 9;8
Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z.
Goldman JA, Garlick JD, Kingston RE
The Journal of biological chemistry 2010 Feb 12;285(7):4645-51
The Journal of biological chemistry 2010 Feb 12;285(7):4645-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BPTF/FALZ Antibody [NB100-41418] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-41418 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BPTF/FALZ Antibody [NB100-41418] - Detection of Human FALZ/BPTF by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BPTF/FALZ Antibody [NB100-41418] - Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for IP at 6 ug per reaction. For blotting immunoprecipitated FALZ/BPTF, NB100-41418 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NB100-41418] - Human ovarian carcinoma. Antibody used at a dilution of 1:200.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NB100-41418] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-FALZ/BPTF used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NB100-41418] - Serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FALZ/BPTF Cat. No. Lot1 used at a dilution of 1:250 (0.8ug/ml) (left) and Cat. No. Lot2 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB.