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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- R33112 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ADAM12 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids AARPLPVSPARALC were used as the immunogen for this ADAM12 antibody. (1)
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the ADAM12 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human heart lysate with ADAM12 antibody at 0.1ug/ml. Predicted molecular weight: ~99/80kDa (long/short form), but observed at ~90/68kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human A431 cells with ADAM12 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate with ADAM12 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placenta with ADAM12 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HeLa cells with ADAM12 antibody (blue) at 10ug/10^6 cells in 0.2ml and naive goat Ig (black).
