Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 19218-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SKIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: YLGTPEEKK LKIILEEMKE KFSMRSGKRN QSKTDAPSGM ELQSWYPVIK QEGDHVSQTH SFLHPSYYLY MCDKVVAPNV SLTSAVSQSK ELTKTEASKS ISRQSEKAHS SGKLQKTVSY PDVSLEEQEK MDLKTSRELC SRLDASISNN STSKRKSESA TCNLVRDINK VGIGLVAAAS SPLLVKDVIC EDDKGKIMEE VMRTYLKQQE KLNLILQKKQ QLQMEVKMLS SSKSMKELTE EQQNLQKELE SLQNEHAQRM EEFYVEQKDL EKKLEQIMKQ KCTCDSNLEK DKEAEYAGQL AELRQRLDHA EADRQELQDE LRQEREARQK LEMMIKELKL QILKSSKTAK E (335-684 aa encoded by BC059386)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (Product # 19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SKIL (IP:19218-1-AP, 4 ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600 ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug.
