Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 10705-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10705-1-AP, RRID:AB_513966
- Product name
- NELFE antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated NELFE antibody (Cat. #10705-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.
Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve.
Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.
RNA polymerase II pausing factor NELF in CD8(+) T cells promotes antitumor immunity.
The RNA-Binding Protein NELFE Promotes Gastric Cancer Growth and Metastasis Through E2F2.
Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag.
Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.
The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.
Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.
Negative elongation factor is essential for endometrial function.
Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.
Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.
Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.
Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.
Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.
Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.
Pan H, Cheng X, Rodríguez PFG, Zhang X, Chung I, Jin VX, Li W, Hu Y, Li R
The Journal of biological chemistry 2023 Nov;299(11):105259
The Journal of biological chemistry 2023 Nov;299(11):105259
Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve.
Chignon A, Argaud D, Boulanger MC, Mkannez G, Bon-Baret V, Li Z, Thériault S, Bossé Y, Mathieu P
PLoS genetics 2022 Jan;18(1):e1010010
PLoS genetics 2022 Jan;18(1):e1010010
Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.
Ou J, Guan X, Wang J, Wang T, Zhang B, Li R, Xu H, Hu X, Guo XK
Mucosal immunology 2022 Feb;15(2):279-288
Mucosal immunology 2022 Feb;15(2):279-288
RNA polymerase II pausing factor NELF in CD8(+) T cells promotes antitumor immunity.
Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, Hu Y, Zhang N, Li R
Nature communications 2022 Apr 20;13(1):2155
Nature communications 2022 Apr 20;13(1):2155
The RNA-Binding Protein NELFE Promotes Gastric Cancer Growth and Metastasis Through E2F2.
Chen C, Zheng Q, Pan S, Chen W, Huang J, Cao Y, Tu Y, Li Z, Yu C, Jie Z
Frontiers in oncology 2021;11:677111
Frontiers in oncology 2021;11:677111
Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag.
Li Y, Nakka K, Olender T, Gingras-Gelinas P, Wong MM, Robinson DCL, Bandukwala H, Palii CG, Neyret O, Brand M, Blais A, Dilworth FJ
STAR protocols 2021 Sep 17;2(3):100751
STAR protocols 2021 Sep 17;2(3):100751
Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.
Robinson DCL, Ritso M, Nelson GM, Mokhtari Z, Nakka K, Bandukwala H, Goldman SR, Park PJ, Mounier R, Chazaud B, Brand M, Rudnicki MA, Adelman K, Dilworth FJ
Developmental cell 2021 Apr 5;56(7):1014-1029.e7
Developmental cell 2021 Apr 5;56(7):1014-1029.e7
The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.
Liu B, Xu Q, Wang Q, Feng S, Lai F, Wang P, Zheng F, Xiang Y, Wu J, Nie J, Qiu C, Xia W, Li L, Yu G, Lin Z, Xu K, Xiong Z, Kong F, Liu L, Huang C, Yu Y, Na J, Xie W
Nature 2020 Nov;587(7832):139-144
Nature 2020 Nov;587(7832):139-144
Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.
Yu L, Zhang B, Deochand D, Sacta MA, Coppo M, Shang Y, Guo Z, Zeng X, Rollins DA, Tharmalingam B, Li R, Chinenov Y, Rogatsky I, Hu X
Nature communications 2020 May 8;11(1):2286
Nature communications 2020 May 8;11(1):2286
Negative elongation factor is essential for endometrial function.
Hewitt SC, Li R, Adams N, Winuthayanon W, Hamilton KJ, Donoghue LJ, Lierz SL, Garcia M, Lydon JP, DeMayo FJ, Adelman K, Korach KS
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):3010-3023
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):3010-3023
Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.
Sacta MA, Tharmalingam B, Coppo M, Rollins DA, Deochand DK, Benjamin B, Yu L, Zhang B, Hu X, Li R, Chinenov Y, Rogatsky I
eLife 2018 Feb 9;7
eLife 2018 Feb 9;7
Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.
Bastide MF, Bido S, Duteil N, Bézard E
Neurobiology of disease 2016 Jan;85:93-98
Neurobiology of disease 2016 Jan;85:93-98
Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
Lu X, Zhu X, Li Y, Liu M, Yu B, Wang Y, Rao M, Yang H, Zhou K, Wang Y, Chen Y, Chen M, Zhuang S, Chen LF, Liu R, Chen R
Nucleic acids research 2016 Aug 19;44(14):6853-67
Nucleic acids research 2016 Aug 19;44(14):6853-67
Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.
Gupte R, Muse GW, Chinenov Y, Adelman K, Rogatsky I
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 3;110(36):14616-21
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 3;110(36):14616-21
Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.
Sawarkar R, Sievers C, Paro R
Cell 2012 May 11;149(4):807-18
Cell 2012 May 11;149(4):807-18
Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.
Iida M, Iizuka N, Tsunedomi R, Tsutsui M, Yoshida S, Maeda Y, Tokuhisa Y, Sakamoto K, Yoshimura K, Tamesa T, Oka M
Oncology reports 2012 Aug;28(2):728-34
Oncology reports 2012 Aug;28(2):728-34
Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.
Adelman K, Kennedy MA, Nechaev S, Gilchrist DA, Muse GW, Chinenov Y, Rogatsky I
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18207-12
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18207-12
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10705-1-AP(RDBP antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The NELFE antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NELFE. This antibody recognizes human,mouse,rat antigen. The NELFE antibody has been validated for the following applications: ELISA, WB, IHC analysis.