PAB20363

antibody from Abnova Corporation

Targeting: NELFE D6S45, NELF-E, RD, RDBP, RDP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20363

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20363, RRID:AB_10964934

Product name

RDBP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RDBP.

Antigen sequence

LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATE
QAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKD
PEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLY
ESFVSSSDRLRELGPDG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RDBP polyclonal antibody (Cat # PAB20363) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with RDBP polyclonal antibody (Cat # PAB20363) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with RDBP polyclonal antibody (Cat # PAB20363) shows strong nuclear positivity in neuronal cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)