A301-914A

antibody from Invitrogen Antibodies

Targeting: NELFE D6S45, NELF-E, RD, RDBP, RDP

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-914A

Provider

Invitrogen Antibodies

Product name

NELFE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NELFE by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NELFE (Cat. No. A301-914A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NELFE by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NELFE antibody A301-914A used for IP at 3 µg/mg lysate. NELFE was also immunoprecipitated by rabbit anti-NELFE antibody A301-913A, which recognizes an upstream epitope. For blotting immunoprecipitated NELFE, A301-914A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.