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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP1-05978 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05978, RRID:AB_1555342
- Product name
- Rabbit Polyclonal NELF-E Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NELF-E Antibody [NBP1-05978] - Detection of whole cell lysate from HeLa (15 & 50 ug), 293T (50 ug) and Jurkat (50 ug) cells, with diluted at 0.4 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NELF-E Antibody [NBP1-05978] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NELFE antibody used for IP at 3 mcg/mg lysate. NELFE was also immunoprecipitated by rabbit anti-NELFE antibody NBP1-05979, which recognizes a downstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NELF-E Antibody [NBP1-05978] - Detection of human NELFE by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NELFE antibody A301-913A (lot A301-913A-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NELFE was also immunoprecipitated by a previous lot (lot A301-913A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
