PA5-52453

antibody from Invitrogen Antibodies

Targeting: CRLF3 CREME9, CYTOR4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52453

Provider

Invitrogen Antibodies

Product name

CRLF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: IEHGVNTAED LVREGEIAML GGVGEENEKL WSFTKKASHI QLDSLPEVPL LVDVPCLSAQ LDDSILNIVK DHIFKHGTVA SRPPVQIEEL IEKPGGIIVR WCKVDDDFTA QDYRLQFRKC TSNHFEDVYV GSE Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 89%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRLF3 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a CRLF3 Polyclonal Antibody (Product # PA5-52453).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRLF3 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a CRLF3 Polyclonal Antibody (Product # PA5-52453). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CRLF3 in human cell line A-431 shows positivity in plasma membrane & cytoplasm. Samples were probed using a CRLF3 Polyclonal Antibody (Product # PA5-52453).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CRLF3 in human lymph node shows strong cytoplasmic positivity in reaction center cells, lymphoid cells outside reaction centra. Samples were probed using a CRLF3 Polyclonal Antibody (Product # PA5-52453).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CRLF3 in human lymph node and pancreas tissues using CRLF3 Polyclonal Antibody (Product # PA5-52453). Corresponding CRLF3 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CRLF3 in human pancreas using CRLF3 Polyclonal Antibody (Product # PA5-52453) shows low expression as expected.