MA1-045

antibody from Invitrogen Antibodies

Targeting: DLG4 PSD-95, PSD95, SAP-90, SAP90

Provider product page for MA1-045

Western blot

Immunocytochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [326]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [5]
Flow cytometry [2]
Other assay [5]

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Product number: MA1-045 - Provider product page
Provider: Invitrogen Antibodies
Product name: PSD-95 Monoclonal Antibody (6G6-1C9)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA1-045 has been successfully used in immunofluorescence analysis of PSD95 in human motor neurons derived from iPSCs.
Antibody clone number: 6G6-1C9
Concentration: 3.3 mg/mL

DLG4 protein structure - MA1-045 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) (Product # MA1-045) shows staining in C6 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 (Product # MA1-045) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) (Product # MA1-045) shows staining in HeLa cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 (Product # MA1-045) at a dilution of 1:100-1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) (Product # MA1-045) shows staining in U251 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 (Product # MA1-045) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PSD95 (green) in human motor neurons derived from iPSCs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 10 minutes, and blocked with 5% donkey serum in PBS for 15 minutes at room temperature. Cells were stained with a PSD95 monoclonal antibody (Product # MA1-045) diluted at 1:500 in 5% donkey serum overnight at 4°C, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature (green). Note: Data courtesy of Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated and cultured using the Primary Neuron Isolation Kit (Product # 88280). At day 28, neurons were fixed with 4% paraformaldehyde, permeablilized with 0.1% triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PSD95 monoclonal antibody (Product # MA1-045) at a dilution of 1:500 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at dilution of 1:500 for 1 hour at room temperature. Images were taken at 20X magnification on a Carl Zeiss microscopy (AxioVision Rel. 4.7).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. MPP2 interacts with the N-terminal domain of SK2-L. ( A ) Co-immunoprecipitations. Western blots prepared from HEK cell lysates expressing SK2-S and SK2-L, either alone (mock) or together with C8-MPP2, immunoprecipitated with either anti-SK2 antibody or IgG (control), and probed for C8-MPP2. Adjacent blot shows input of C8-MPP2. ( B ) A similar experiment except using PSD-95 instead of C8-MPP2. PSD-95 is expressed but does not co-IP with SK2. ( C ) GST-pull-downs. Blot probed with anti-His antibody shows input prey proteins, His-SK2-L N-terminal domain and His-PSD-93. After exposure to GST-baits representing SH3-HOOK-GK domains of SAP97, CaCNB4, or MPP2, the His-SK2-L N-terminal domain was specifically retained by GST-MPP2. Positive control shows interaction between GST-C-terminal domain of Kv1.4 and His-PSD-93. Co-immunoprecipitations and GST-pull-downs were performed in triplicate. DOI: http://dx.doi.org/ Figure 2--figure supplement 1. Co-immunoprecipitations. ( A ) Immunoprecipitation and input of SK2-S plus SK2-L. Western blot probed with anti-SK2 antibody raised in rabbit. First three lanes show immunoprecipitates from transfected HEK293 cell lysates co-transfected with empty vector (mock), C8-MPP2, or PSD-95 (see Figure 2 ). Immunoprecipitations were performed using anti-SK2 antibody raised in guinea pig. Last three lanes show input material prior to immunoprecipitation. ( B ) Co-immunoprecipitation of C8-SAP-97 co-expressed in HEK293 cells with GluA1 but not

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Object recognition training induces an increase in the expression of Arc, alpha -CaMKII, and PSD-95 proteins in the dentate gyrus. Representative blots and comparisonof normalized protein levels of (a) Arc, (b) alpha -CaMKII, and (c) PSD-95 proteins are presented for the C60 and L60 groups (relative to CC). Data arepresented as mean +- SEM ( n = 7 for all groups). Protein levels were normalized to beta -actin. Asterisks indicate P