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LearnNB300-556
antibody from Novus Biologicals
Targeting: DLG4
PSD-95, PSD95, SAP-90, SAP90
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- NB300-556 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-556, RRID:AB_2092366
- Product name
- Mouse Monoclonal PSD-95 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Detects Post Synaptic Density 95 kDa (PSD-95) from rat tissues.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 uL
- Concentration
- 3.3 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Sustained neuronal and microglial alterations are associated with diverse neurobehavioral dysfunction long after experimental brain injury.
The effects of social instability stress and subsequent ethanol consumption in adolescence on brain and behavioral development in male rats.
Adolescent social instability stress alters markers of synaptic plasticity and dendritic structure in the medial amygdala and lateral septum in male rats.
High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons.
Sex-specific effects of CB1 receptor antagonism and stress in adolescence on anxiety, corticosterone concentrations, and contextual fear in adulthood in rats.
Effects of CB1 receptor antagonism and stress exposures in adolescence on socioemotional behaviours, neuroendocrine stress responses, and expression of relevant proteins in the hippocampus and prefrontal cortex in rats.
SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus.
Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits.
pT305-CaMKII stabilizes a learning-induced increase in AMPA receptors for ongoing memory consolidation after classical conditioning.
Group I metabotropic glutamate autoreceptors induce abnormal glutamate exocytosis in a mouse model of amyotrophic lateral sclerosis.
Aplysia synapse associated protein (APSAP): identification, characterization, and selective interactions with Shaker-type potassium channels.
SynCAMs organize synapses through heterophilic adhesion.
Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus.
Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus.
Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family.
Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family.
Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.
Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies.
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies.
Ritzel RM, Li Y, He J, Khan N, Doran SJ, Faden AI, Wu J
Neurobiology of disease 2020 Mar;136:104713
Neurobiology of disease 2020 Mar;136:104713
The effects of social instability stress and subsequent ethanol consumption in adolescence on brain and behavioral development in male rats.
Marcolin ML, Baumbach JL, Hodges TE, McCormick CM
Alcohol (Fayetteville, N.Y.) 2020 Feb;82:29-45
Alcohol (Fayetteville, N.Y.) 2020 Feb;82:29-45
Adolescent social instability stress alters markers of synaptic plasticity and dendritic structure in the medial amygdala and lateral septum in male rats.
Hodges TE, Louth EL, Bailey CDC, McCormick CM
Brain structure & function 2019 Mar;224(2):643-659
Brain structure & function 2019 Mar;224(2):643-659
High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons.
Parker SS, Moutal A, Cai S, Chandrasekaran S, Roman MR, Koshy AA, Khanna R, Zinsmaier KE, Mouneimne G
eNeuro 2018 Sep-Oct;5(5)
eNeuro 2018 Sep-Oct;5(5)
Sex-specific effects of CB1 receptor antagonism and stress in adolescence on anxiety, corticosterone concentrations, and contextual fear in adulthood in rats.
Simone JJ, Baumbach JL, McCormick CM
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2018 Oct;69:119-131
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2018 Oct;69:119-131
Effects of CB1 receptor antagonism and stress exposures in adolescence on socioemotional behaviours, neuroendocrine stress responses, and expression of relevant proteins in the hippocampus and prefrontal cortex in rats.
Simone JJ, Baumbach JL, McCormick CM
Neuropharmacology 2018 Jan;128:433-447
Neuropharmacology 2018 Jan;128:433-447
SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus.
Hussain S, Ringsevjen H, Egbenya DL, Skjervold TL, Davanger S
Frontiers in molecular neuroscience 2016;9:10
Frontiers in molecular neuroscience 2016;9:10
Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits.
Hussain S, Davanger S
PloS one 2015;10(10):e0140868
PloS one 2015;10(10):e0140868
pT305-CaMKII stabilizes a learning-induced increase in AMPA receptors for ongoing memory consolidation after classical conditioning.
Naskar S, Wan H, Kemenes G
Nature communications 2014 May 30;5:3967
Nature communications 2014 May 30;5:3967
Group I metabotropic glutamate autoreceptors induce abnormal glutamate exocytosis in a mouse model of amyotrophic lateral sclerosis.
Giribaldi F, Milanese M, Bonifacino T, Anna Rossi PI, Di Prisco S, Pittaluga A, Tacchetti C, Puliti A, Usai C, Bonanno G
Neuropharmacology 2013 Mar;66:253-63
Neuropharmacology 2013 Mar;66:253-63
Aplysia synapse associated protein (APSAP): identification, characterization, and selective interactions with Shaker-type potassium channels.
Reissner KJ, Boyle HD, Ye X, Carew TJ
Journal of neurochemistry 2008 May;105(3):1006-18
Journal of neurochemistry 2008 May;105(3):1006-18
SynCAMs organize synapses through heterophilic adhesion.
Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Nov 14;27(46):12516-30
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Nov 14;27(46):12516-30
Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus.
Zhang W, Vazquez L, Apperson M, Kennedy MB
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Jan 1;19(1):96-108
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Jan 1;19(1):96-108
Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus.
Furuyashiki T, Fujisawa K, Fujita A, Madaule P, Uchino S, Mishina M, Bito H, Narumiya S
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Jan 1;19(1):109-18
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Jan 1;19(1):109-18
Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family.
Gomperts SN
Cell 1996 Mar 8;84(5):659-62
Cell 1996 Mar 8;84(5):659-62
Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family.
Gomperts SN
Cell 1996 Mar 8;84(5):659-62
Cell 1996 Mar 8;84(5):659-62
Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.
Kornau HC, Schenker LT, Kennedy MB, Seeburg PH
Science (New York, N.Y.) 1995 Sep 22;269(5231):1737-40
Science (New York, N.Y.) 1995 Sep 22;269(5231):1737-40
Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.
Kornau HC, Schenker LT, Kennedy MB, Seeburg PH
Science (New York, N.Y.) 1995 Sep 22;269(5231):1737-40
Science (New York, N.Y.) 1995 Sep 22;269(5231):1737-40
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies.
Patton BL, Molloy SS, Kennedy MB
Molecular biology of the cell 1993 Feb;4(2):159-72
Molecular biology of the cell 1993 Feb;4(2):159-72
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies.
Patton BL, Molloy SS, Kennedy MB
Molecular biology of the cell 1993 Feb;4(2):159-72
Molecular biology of the cell 1993 Feb;4(2):159-72
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 Double labeling with antibodies against syntaxin-1 (D) and synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in Neuro-2a cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in SH-SY5Y cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in U87-MG cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
